December 2014 Issue

Analyze this Dec

CIL SILAC Protein Quantitation Kit

CIL


Stable isotope labeling by amino acids in cell culture (SILAC) is a simple, robust, yet powerful approach for in vivo incorporation of a label into proteins for mass spectrometry (MS)-based quantitative proteomics.

SILAC relies on metabolic incorporation of a given ‘light’ or ‘heavy’ form of the amino acid into the proteins. The method relies on the incorporation of amino acids with substituted stable isotopic nuclei.

This chemical labeling technology for protein identification and mass analysis by liquid chromatography and mass spectrometry (LC-MS); includes stable isotope labeling reagents.

CIL offers complete reagent kits, media, as well as mass variants of lysine, arginine and other amino acid for analysis of protein expression by mass spectrometry using SILAC.

Click here to view the SILAC product listing from CIL.

Click here for the literature reference.

Dedicated Benches for Mass Spectrometry

Ion Bench

IONBENCH manufactures and delivers mobile benches, especially designed for Mass Spectrometers.

  • Noise reduction performance : – 15 db(A) (75% reduction in noise perception)
  • Housing one or two vacuum pumps
  • Movable bench to get an easy access to the rear of the instruments
  • Thick special acoustic foam covers the interior of the cabinet
  • Noise reduction cabinet integrates an audible & visual (red LED) overheating temperature alarm (on battery backup) for an increased vacuum pump lifetime.

Vibration

  • High quality vibration patented dampening system avoids vibrations being telegraphed from the primary vacuum pumps to the Mass Spectrometer (especially to the Turbomolecular pump inside the MS)
  • Also available : optional information control package, CPU holder, dedicated solvent waste storage, oil drain pump, …

Click here to check your MS configuration.

Visit us at 8th Singapore International Chemistry Conference 2014 to view the demo unit. Click here for details.

Chromsystems kits for clinical diagnostics

Chrom cert

Chromsystems is a leading global company providing ready-to-use reagent kits and supplies for routine clinical diagnostics by high performance liquid chromatography and tandem mass spectrometry. The product portfolio includes complete kits as well as quality controls and calibrators, all ensuring highly accurate as well as cost-effective analysis in the laboratory. Chromsystems‘ products are also CE-IVD compliant, satisfying regulatory requirements for the laboratory.

Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism and in stress response. The determination of parameters is vital for the diagnosis of a number of diseases such as adrenogenital syndrome, tumours or hyperaldosteronism.

The MassChrom® Steroid reagent kit allows the quantitative determination of 13 steroid hormones in serum/plasma by LC-MS/MS and includes the clinically most important parameters. Sample preparation is the same for both steroid panels using a solid phase extraction in 96 SPE well plates. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method also does not require a high-end LC-MS/MS system to be carried out.

Read more about MassChrom® Steroids.

Newborn screening (NBS) is the practice of testing every newborn for certain harmful or potentially fatal disorders that are not otherwise apparent at birth. In general, disorders related to protein and fat metabolism can hinder an infant’s normal physical and mental development in a variety of ways.

The benefits to society of early diagnosis of these disorders are considerable; initiation of treatment prior to onset of symptoms can avoid permanent neurological impairment and more importantly saves lives.

The Chromsystems reagent kit for the determination of amino acids and acylcarnitines from dried blood spot samples using tandem mass spectrometry is intended for the early diagnosis of amino acid and fatty acid metabolic disorders during newborn screening.

  • Ready-to-use kit, includes all reagents and quality controls
  • Dried Blood Spot Controls
  • Reagents stable after reconstitution (min. 3 weeks refrigerated)
  • Fast sample preparation: 20 min no derivatization, 95 min incl. succinylacetone, 60 min excl. succinylacetone
  • Tuning mix available
  • 27 isotopically labelled internal standards including Arg, Asp, Glu and Orn
  • No carcinogens during sample preparation

Read more about MassChrom® Amino Acids and Acylcarnitines.

Vitamin D has been widely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects.

25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-Vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation.

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry.

  • Isotopically labelled internal standard
  • Multilevel calibrators
  • Online sample preparation
  • Upgrade available for the dermination of 3-epi-25-OH-Vitamin D3/D2

Read more about MassChrom® 25-OH-Vitamin D3