June 2014 Issue

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CCK-8 for Natural Products Analysis in Treatment of Cancer

CCK8

Thousands of different species of plant have been reported as being useful for the treatment of cancer. There has been an argument that the use of natural products has been the single most successful strategy in the discovery of novel medicines. Tulp, Martin; Bohlin, Lars (2002)

Dojindo’s Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays of your natural product analysis.

Benefits of CCK-8:

  • No Thawing Necessary- 3 simple steps
  • Stable One Bottle Solution, at 4ºC for 1 year
  • More Sensitive than MTT, MTS, or WST-1
  • No Toxicity to Cell

Refer below for some publications related to CCK-8 used in natural products analysis for cancer treatment:

1. Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro

This study was conducted to evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231. The sample was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days before cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules were conducted. . Read more

2. Sulforaphane induces cell cycle arrest by protecting RB-E2F-1 complex in epithelial ovarian cancer cells

Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. This paper is a study conducted to understand the signaling mechanisms through which SFN influences the cell growth and proliferation in epithelial ovarian cancer cells (EOC). Cell proliferation assays were conducted using Cell Counting Kit-8 (CCK-8). Read more

Click here to watch a video to learn more about CCK-8.

Request a free sample CCK-8. Write to us at marketing@analisa-scientific.com

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Mechanical Homogenization using Mini-GTM

Mini G

Increasing concern over the health effects of residual pesticides on fruits and vegetables has led to increased testing of these products to determine the levels of pesticides on produce when it goes to market. Since its introduction in 2003 by Anastassiades and Lehotay et al, the QuEChERS method (quick, easy, cheap, effective, rugged, safe) has proven to be effective and convenient for analysis of multiple pesticides in agricultural products.

In the Sample Preparation stage, the measured pesticide concentration can be greater when a mechanical grinder is used to shake the samples compared to traditional manual shaking method.

Due to the more rapid and vigorous agitation using the Spex SamplePrep’s grinder, mixing should be more thorough than can be achieved manually and it is not surprising that extraction is improved over the manual method.

The addition of two ceramic grinding cylinders to each tube shaken on the grinder also aids the extraction process. The ceramic cylinders mash and grind the fruit or vegetable matrix further during the shaking process, thereby increasing surface area and allowing a more complete extraction of pesticide from the matrix.

In fact, the matrices in the samples shaken on Spex SamplePrep’s grinder visibly appeared more thoroughly ground than samples shaken by hand.

The MiniG™ is the ideal solution for labs that want a compact yet powerful tissue homogenizer. It has been created for applications requiring sample preparation for DNA/RNA and protein extractions as well as pesticide residue analysis.It is affordable, gives you medium throughput and provides consistency in your sample preparation method.

Learn more about Spex SamplePrep’s MiniG™ for your natural products analysis

Apolipoprotein A-1 (ApoA-1) to determine risk for developing cardiovascular disease

APO

Apolipoprotein A-1 (ApoA-1) is the primary protein component of high-density lipoprotein (HDL) in plasma and has a specific role in lipid metabolism. It is a structural and functional protein that promotes cholesterol efflux from tissues to the liver.

Testing for ApoA-1 is used to approximate HDL levels in a subject, which can be used to help determine a person’s risk for developing cardiovascular disease. By combining the power of LC/MS and a heavy-labeled ApoA-1 internal standard, accurate quantitation of this protein in a biological sample is achievable using a bottom-up proteomic workflow.

Read more.

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