Mechanical Homogenization using Mini-GTM
Increasing concern over the health effects of residual pesticides on fruits and vegetables has led to increased testing of these products to determine the levels of pesticides on produce when it goes to market. Since its introduction in 2003 by Anastassiades and Lehotay et al, the QuEChERS method (quick, easy, cheap, effective, rugged, safe) has proven to be effective and convenient for analysis of multiple pesticides in agricultural products.
In the Sample Preparation stage, the measured pesticide concentration can be greater when a mechanical grinder is used to shake the samples compared to traditional manual shaking method.
Due to the more rapid and vigorous agitation using the Spex SamplePrep’s grinder, mixing should be more thorough than can be achieved manually and it is not surprising that extraction is improved over the manual method.
The addition of two ceramic grinding cylinders to each tube shaken on the grinder also aids the extraction process. The ceramic cylinders mash and grind the fruit or vegetable matrix further during the shaking process, thereby increasing surface area and allowing a more complete extraction of pesticide from the matrix.
In fact, the matrices in the samples shaken on Spex SamplePrep’s grinder visibly appeared more thoroughly ground than samples shaken by hand.
The MiniG™ is the ideal solution for labs that want a compact yet powerful tissue homogenizer. It has been created for applications requiring sample preparation for DNA/RNA and protein extractions as well as pesticide residue analysis.It is affordable, gives you medium throughput and provides consistency in your sample preparation method.
Learn more about Spex SamplePrep’s MiniG™ for your natural products analysis
Apolipoprotein A-1 (ApoA-1) to determine risk for developing cardiovascular disease
Apolipoprotein A-1 (ApoA-1) is the primary protein component of high-density lipoprotein (HDL) in plasma and has a specific role in lipid metabolism. It is a structural and functional protein that promotes cholesterol efflux from tissues to the liver.
Testing for ApoA-1 is used to approximate HDL levels in a subject, which can be used to help determine a person’s risk for developing cardiovascular disease. By combining the power of LC/MS and a heavy-labeled ApoA-1 internal standard, accurate quantitation of this protein in a biological sample is achievable using a bottom-up proteomic workflow.