September 2014 Issue

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September 2014 Issue

High Sensitivity and Optimized method for shrimp pathogens detectionShrimp

Optimized Real-Time PCR workflows combining sample preparation and amplification solution

a) Sample preparation of shrimp tissues

  • tissue homogenization with bead beating

b) Nucleic acid extraction and purification

  • Using optimized magnetic beats protocols
  • Manual extractions or options with automate MagMAX™ Express-24 (24 samples) and MagMAX™ Express -96 (96 samples) instrument

c) Amplification and detection with Real-time PCR

  • Determination of shrimp pathogen including:

- white spot syndrome virus (WSSV)
- yellow head virus (THV)
- Taura syndrome virus (TSV)
- infectious myonecrosis virus (IMNV)
- infectious hypodermal and hematopoietic -necrosis virus (IHHNV)

Benefits of Real-time PCR method:

  • internal positive control (IPC), which enables the monitoring of nucleic acid isolation efficacy as well as the detection of PCR inhibition
  • Speed : 15 hours run on Real-time PCR system; get your results on the same day
  • Higher sensitivity & Specificity than conventional PCR with LOD less than 25 copies or equal to 50 copies
  • Able to multiplex few pathogens into single tube detection (Multiplex assay will be available soon)
  • High throughput (up to 96 reactions/run) & ease of handling with minimal hands on time compared to conventional PCR
  • Reduce risk of contamination

Click here to find out more about Life Technologies solutions on Shrimp diseases.

The first USDA-licensed, PCR-based solution for the detection of avian influenza

Avian

In keeping with our continued development of next-generation molecular tools, we’re proud to announce the availability of our USDA-licensed VetMAX™-Gold AIV Detection Kit: a rapid PCR-based testing solution that detects avian influenza with a high level of specificity, sensitivity, and reproducibility.

Avian influenza (AI) is a highly contagious viral disease that can infect several species of birds, such as chickens, turkeys, quails, guinea fowls, and ducks. It is caused by an A-type influenza virus: a single-stranded RNA virus belonging to the orthomyxoviridae family. AI can present many signs in birds, from a minor disease with few or no clinical signs, to a disease that can quickly become fatal and lead to a serious epidemic.

Low pathogenicity AI viruses can cause various clinical problems ranging from infections to drops in egg production. Highly pathogenic avian influenza belonging to H5, H7, and H9 subtypes can cause severe disease with high morbidity and mortality rates in commercial poultry operations. Virus transmission between birds occurs mainly by respiratory secretion and fecal material, but can also happen indirectly, through bird droppings carrying the virus, or contaminated food, water, and materials.

Read more.

Improved quality and less degradation on isolation of total RNA from small marine mammal tissue samples using SPEX Freezer/Mill

Mammal

The Voyage of the Odyssey by Ocean Alliance was launched to address integrated dataset allowing a consistent analysis of exposure to, and potential effects of, persistent organochlorines and other pollutants in marine life.

Marine mammals can be considered environmentally relevant candidates for use as sentinel species when assessing marine pollution. The sperm whale (Physeter macrocephalus) was chosen as the indicator species for the Voyage of the Odyssey. A total of 960 sperm whale skin and blubber samples and over 100 samples from predatory fish were collected during the voyage.

As part of the project, a biomarker study was conducted focusing on the use of the cytochrome P450 1 (CYP1) enzymes. CYP1 enzymes can metabolize and/or activate environmental pollutants, such as polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). In many animal species CYP1 induction is used as a biomarker of exposure and molecular effects to the above contaminants. The goal of the project is the investigation of the molecular effects of pollution in cetacean samples using the cytochrome P450 CYP 1A1, 1A2, and 1B1 as biomarkers.

Methods include isolating RNA sequencing, cloning and quantifying the expression of the 3 CYP genes in sperm whale skin biopsies. The sperm whale skin samples available for this particular biomarker project weigh only between 50-100ug. The amount of total RNA that can be extracted from such small samples is low; therefore the use of the SPEX Freezer/Mill in order to increase the quantity of total RNA that can be extracted from each sample and its quality was investigated. The SPEX Freezer/Mill has been a substantial asset to the research by allowing the isolation of total RNA from small tissues from marine samples with improved quality and less degradation than previous techniques.

Please click here to download the application note.

Animal Feed Analysis- Reference Standards and Columns

Poultry

Animal nutrition constitutes an important issue for the animal production industry. Feed additives are gaining importance due to various functions such as growth promotion, controlling infectious diseases and enhancement of feed digestibility in animals. Products intended for animal feed may contain undesirable substances which could endanger animal health or, because of their presence in livestock products, human health or the environment.

Every sector of the livestock industry, the associated services and the well-being of both animals and humans are influenced by animal feeding. The availability of accurate, reliable and reproducible analytical data is imperative for proper feed formulation. Only reliable analysis can lead to the generation of sound scientific data.

Find out more about our analytical solutions for animal feed analysis:

  • Reference standards from Dr Ehrenstorfer constituting vitamins, amino acids, antibiotics, mycotoxins. Click here to look at the full list
  • C18 Columns from Grace. Download the application note referring Grace HPLC Column in LC-MS method for the determination of the feed additive semduramicin, in poultry feed.

Highlights

Analisa Resources at Malaysian Association of Veterinary Pathology Conference

MAVP

2014 brought us many exciting moments through many exhibitions; Malaysian Association of Veterinary Pathology Conference which took place from 22nd – 23rd August in Lumut, Perak was one of it.
The event provided us the platform to meet many of our valued customers as well as new customers and many fruitful discussions took place.

Get a copy of our latest Life TechnologiesTM Animal Health Molecular Diagnostic Tools product guide by emailing us at marketing@analisa-scientific.com.

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