September 2014 Issue

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The lowest dead volume liquid handler on the market. Stop wasting time and ingredient!

Mantis

Mantis is a low dead-volume, non-contact liquid dispenser. The unique design of this small machine minimizes dead volume and accepts pipette or tube inputs.

  • Clean – The fluid path of the Mantis is both disposable and washable
  • Low Dead Volume – A pipette tip can input directly into the Mantis’ chip to cut dead volumes down to 6μL
  • Flexible – Can dispense up to 6 reagents automatically with optional chip changer and can accept most SBS plates as well as deep well blocks
  • Reliable – Dispenses using our robust non-contact microdiaphragm pump technology via positive displacement with no risk of clogging even with difficult to dispense reagents
  • Chemical Compatibility – wide variety of chips are available for dispense needs including perfluoroelastomer for aggressive solvents, as well as molecular biology grade chips free of detectable DNA, DNase, RNase and Protease

Applications:

  • Assay Development
  • PCR Setup
  • Cell Dispensing
  • Precious Reagent Dispensing


Click here to watch a video on the performance of the Mantis system

Shodex Polymer Columns for Saccharide analysis

Sugar Shodex

The term “Saccharide” generally refers to simple saccharides, oligosaccharides, polysaccharides, sugar alcohols and the like. Simple saccharides like monosaccharides and disaccharides, exclude sugar alcohols. Emphasized labeling such as, “Very Low Sugar” or “Low sugar” and “Sugarless” is not permitted in some countries unless the content of the relevant type of sugar is not more than 5 grams per 100 grams (2.5 g/100 mL for refreshing beverages), and less than 0.5 grams per 100 grams (0.5 g/100 mL for refreshing beverages), respectively.

Shodex is world leading provider of Polymer Based HPLC columns and provides many kind of columns for saccharide analysis. One of it is Shodex Asahipak NH2P-50 4E.

Click here to view all the columns from Shodex for saccharide analysis.

Purification of Phenolic Compound from Curcuma longa (Turmeric) Using Mass Directed Fractionation

Purion

The purification of natural products remains challenging, lengthy, and tedious. Automated flash chromatography instruments ease this process.

Reversed Phase Liquid Chromatography-Mass Spectrometry (LC-MS) has become a routine technique in today’s laboratory. A study involving an experimental Flash Chromatography-
Mass Spectrometry (FCMS) system, CombiFlash® Rf+ PurIon™ was used with both normal and reversed phase solvent systems to screen for novel compounds in Curcuma longa (turmeric) during purification.

Curcuma longa is valued for the yellow color it imparts to food, and has also been implicated in wound healing, anti-oxidant, anti-inflammatory, anti-carcinogenic effects and natural coloring activities.

From this study, it has been proven that by using a mass spectrometer as a detector, potentially interesting compounds can be easily identified, prioritized, and purified in sufficient quantities to allow evaluation for biological activity.

The system can be run either in SIC mode to capture a particular compound in a mixture or in XIC to purify complex mixtures from natural products extracts. In either mode, the system collects the entire mass spectrum so that the other compounds can be identified.

Please click here to download the application note.

Optimized DNA Extraction from Basil lysed with Geno Grinder

Geno Basil

Effective cell disruption and DNA/RNA extraction are critical for natural products research. SPEX SamplePrep’s Geno Grinder aids your research by providing high-throughput sample preparation solutions.

Geno Grinder functions as mechanical cell lysers used for effective extraction of genomic DNA from samples containing plant tissue. However, all mechanical lysers do not provide the same outcome.

A study was performed to discover whether Geno/Grinder processing variables significantly increased or decreased the yields of genomic DNA in fresh basil. Variables investigated included homogenization time and speed, while buffers, grinding media, and tubes were constant. In this experiment, the Geno Grinder returned most results higher than 6,557bp meaning that significant shearing or other degradation did not occur even with longer processing times.

The 2000 Geno/Grinder® was compared with a competitive cell lyser (Competitor A) for extraction of DNA from fresh basil. Results indicated that use of the Geno/Grinder provided DNA with higher molecular weight than the competitive instrument. In addition, optimal homogenization conditions using the Geno/Grinder were found to be 90 sec. at a rate of 2000 cyc/min.

Download the application note.

Vydac 150HC Peptide Media for Peptide Therapeutics
manufacturing