Purification of Phenolic Compound from Curcuma longa (Turmeric) Using Mass Directed Fractionation
The purification of natural products remains challenging, lengthy, and tedious. Automated flash chromatography instruments ease this process.
Reversed Phase Liquid Chromatography-Mass Spectrometry (LC-MS) has become a routine technique in today’s laboratory. A study involving an experimental Flash Chromatography-
Mass Spectrometry (FCMS) system, CombiFlash® Rf+ PurIon™ was used with both normal and reversed phase solvent systems to screen for novel compounds in Curcuma longa (turmeric) during purification.
Curcuma longa is valued for the yellow color it imparts to food, and has also been implicated in wound healing, anti-oxidant, anti-inflammatory, anti-carcinogenic effects and natural coloring activities.
From this study, it has been proven that by using a mass spectrometer as a detector, potentially interesting compounds can be easily identified, prioritized, and purified in sufficient quantities to allow evaluation for biological activity.
The system can be run either in SIC mode to capture a particular compound in a mixture or in XIC to purify complex mixtures from natural products extracts. In either mode, the system collects the entire mass spectrum so that the other compounds can be identified.
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Optimized DNA Extraction from Basil lysed with Geno Grinder
Effective cell disruption and DNA/RNA extraction are critical for natural products research. SPEX SamplePrep’s Geno Grinder aids your research by providing high-throughput sample preparation solutions.
Geno Grinder functions as mechanical cell lysers used for effective extraction of genomic DNA from samples containing plant tissue. However, all mechanical lysers do not provide the same outcome.
A study was performed to discover whether Geno/Grinder processing variables significantly increased or decreased the yields of genomic DNA in fresh basil. Variables investigated included homogenization time and speed, while buffers, grinding media, and tubes were constant. In this experiment, the Geno Grinder returned most results higher than 6,557bp meaning that significant shearing or other degradation did not occur even with longer processing times.
The 2000 Geno/Grinder® was compared with a competitive cell lyser (Competitor A) for extraction of DNA from fresh basil. Results indicated that use of the Geno/Grinder provided DNA with higher molecular weight than the competitive instrument. In addition, optimal homogenization conditions using the Geno/Grinder were found to be 90 sec. at a rate of 2000 cyc/min.
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